27 to 31 October 2025

Coordinators: Emma Petiot (Université Claude Bernard Lyon), Karen Moreau (Université Claude Bernard Lyon)

Applications will open soon!

The deadline for applications is 16 May.

Acceptance decisions will be announced in early June 2025.

PROGRAMME

Full hands-on program of 4 days

• Knowledge of lab-scale bioreactors (stainless steel / single-use)
  i.e. identification of bioreactor parts / Bioreactor mounting / sterilizing
• Suspension cell culture in bioreactors (CHO, Sf9)
  i.e. comparison of culture conditions (ex. culture medium / infection conditions like MOI screening)
• Standard cell culture monitoring
  Sterile Sampling, Cell counting, identification of viral replication signs / transgene expression, Metabolic analysis / Cell death
• Cell infection vs transduction process
  i.e. kinetics / impacting parameters: MOI / Media / process / scale-up

Please find the programme as pdf file below.

Access to Level 2 laboratories equipped with

• 500 mL stainless steel Bioreactors (Applikon)
• 2 L single-use bioreactor (Sartorius Biostat A )
• 20 L Single-use  Rocker Bioreactor (Cytiva)
• Standard Cell culture equipment’s

MANDATORY PRE-REQUISITE:

• Applying academic or industrial (PhD students, Post-Doc, Engineers, etc.) should already have cell culture knowledge and, ideally, have already followed one of the ESACT theoretical courses.